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The course will start on Sunday evening by informal get-together party. Then on Monday bus transport to BIOCEV research centre will be provided and full day will be spent there with minisymposium on biophysical techniques and their practical aspects, including demos and discussions, so these introductory lectures for the different methodologies will be followed by their experimental aspects explanations. The practical aspects will be covered in a mixture of hands-on exercises and demonstrations. This will take place in laboratories of Centre of Molecular Structure, BIOCEV, alternating with review and discussion of experimental strategies in the seminar room, rotating small groups of students among different techniques. We will review the basic operation of the instruments, choice of buffers, and detection systems, and experimental design dependent on the system under study. For AUC, assembling the AUC cells and operating the XLA/I will be discussed in detail at the Faculty of Science, Charles University within the next part of the course.
The following part of the course, from Tuesday till Friday, will be devoted to understanding the theoretical principles of data analysis of AUC sedimentation experiments and practical data analysis sessions and will take place in a classroom at the Faculty of Science, Charles University. The goal is to ensure through hands-on training that the participants become familiar with the details of the use of the software, and confident with each type of data analysis. We will have a lecture series, to introduce and recapitulate the theoretical background as well as the concepts for their implementation in the software, mixed with practical exercises on examples, where each participant will conduct data analyses on his/her laptop concurrent with the step-by-step demonstration projected on the large screen. Additional lectures on data analysis from other related techniques, i.e., ITC, SPR, and fluorescence will build up on previous skills in data analysis during the course, as well as methods of global analysis of data coming from different techniques.
Transportation to BIOCEV for workshop participants and tutors will be provided on Monday while their accommodation is located near the Faculty of Science, Charles University which is conveniently close to the Prague city center. On Monday evening a poster session with reception dinner will be organized at BIOCEV, on Tuesday, Wednesday and Thursday evening all participants and tutors will be invited for a dinner in the Old Prague and/or good restaurants near the Faculty and hotel.
The practical course will include:
- General presentations and round table discussions emphasizing and illustrating the benefits of transverse approaches
- Theoretical presentations of the techniques, including practical advices for setting up an experiment, possible trouble shooting
- Practical demonstrations for four groups of six students; each group following all the demos
- Data analysis: basics and in-depth focus on SEDFIT/SEDPHAT which are freely available programs developed by Peter Schuck that are extensively used all over the world and allow multi-method global data analysis
- Parallel sessions focused on special topics regarding advanced data analysis
- Individual one-on-one training sessions with the tutors enabling discussion of data analysis of the students’ own data
- Short-talks from students selected from the list of abstracts for poster session
A round table will be organized at the end of the course and a satisfaction survey will be performed afterwards for gathering immediate retrospective feed-backs.
Final Course Agenda:
"Biomolecular Interactions: Methods & Applications" Minisymposium on Biophysical MethodsThe first day of the course will be organized as minisymposium with invited lectures on various biophysical techniques highlighting their usability for biological macromolecule research. Details and programme of the minisymposium are published below.
Selected participants will be offered the possibility to present and discuss their research.
Posters are welcome and will be displayed during the poster session in the beginning of the course.